Spinal cord dorsal horn microdialysis technique, used to monitor extracellular levels of transmitter substances in the central nervous system of laboratory animals as a reflection of transmitter release, is based on the ability of neurotransmitters to diffuse in the extracellular fluid from the site of release and to cross a semipermeable dialysis membrane.

Several neurotransmitters in the CNS (e.g. serotonin, norepinephrine, GABA, opioids, substance P), have been proposed to be involved in the pain-alleviating effect of surface of the spinal cord.

We provide the service of spinal cord dorsal horn microdialysis, in the spinal dorsal horn of rats, and the measurement of neurotransmitters.